Zebrafish gene: strada
Human gene: STRADA (STE20 related adaptor alpha)
Homology score: 68%
ClinVar variants: 7
Gene description: Predicted to have protein serine/threonine kinase activity. Predicted to be involved in several processes, including activation of protein kinase activity; signal transduction by protein phosphorylation; and stress-activated protein kinase signaling cascade. Predicted to localize to cytoplasm. Orthologous to human STRADA (STE20 related adaptor alpha).
ZFIN link: https://zfin.org/ZDB-GENE-030131-6482
Ensembl link: https://uswest.ensembl.org/Danio_rerio/Gene/Summary?db=core;g=ENSDARG00000037929;r=3:31897029-31917390;t=ENSDART00000146275
Zebrafish reference(s): n/a
This gene has 1 transcript (splice variant).
WT DNA sequence (mutation area: exon 6)
Mutant DNA sequence (mutation area: 8 bp deletion in exon 6)
Guide RNA target sites: taatacgactcactataGGTGGAACAGCTTAGACACAgttttagagctagaa
Primers:
Forward primer: cctcaaaattctacactcaataccc
Reverse primer: AGACACTCTTGTACGGGAGG
WT Protein sequence
Representative mutant behavior data
N = 87
