Zebrafish gene: slc6a1b
Human gene: SLC6A1 (solute carrier family 6 member 1)
Homology score: 92%
ClinVar variants: 52
Gene description: Predicted to have gamma-aminobutyric acid:sodium symporter activity and neurotransmitter binding activity. Predicted to be involved in taurine transport. Predicted to localize to axon; cell surface; and plasma membrane. Is expressed in basal plate midbrain region and nervous system. Orthologous to human SLC6A1 (solute carrier family 6 member 1).
ZFIN link: https://zfin.org/ZDB-GENE-041114-57
Ensembl link: https://uswest.ensembl.org/Danio_rerio/Gene/Summary?db=core;g=ENSDARG00000039647;r=11:997100-1024483;t=ENSDART00000017551
Zebrafish reference(s): n/a
This gene has 2 transcripts (splice variants).
WT DNA sequence
Mutant DNA sequence
Guide RNA target sites: taatacgactcactataGGGCGTAGGATGGACTGGAAgttttagagctagaa
Primers:
Forward primer: gcagagcatttatgtgctggc
Reverse primer: atttgaagcctcgactgtagg
WT Protein sequence
N = 84
