Zebrafish gene: prickle1a
Human gene: PRICKLE1 (prickle planar cell polarity protein 1)
Homology score: 85%
ClinVar variants: 3
Gene description: Predicted to have zinc ion binding activity. Involved in several processes, including animal organ development; gastrulation; and generation of neurons. Is expressed in several structures, including central nervous system; ectoderm; eye; mesoderm; and pharyngeal arch. Human ortholog(s) of this gene implicated in progressive myoclonus epilepsy 1B. Orthologous to human PRICKLE1 (prickle planar cell polarity protein 1).
ZFIN link: https://zfin.org/ZDB-GENE-030724-5
Ensembl link: https://uswest.ensembl.org/Danio_rerio/Gene/Summary?db=core;g=ENSDARG00000040649;r=25:314608-348784;t=ENSDART00000059514
Zebrafish reference(s): n/a
This gene has 1 transcript (splice variant).
WT DNA sequence (mutation area: exon 2)
Mutant DNA sequence (mutation area: 1 bp deletion in exon 2)
Guide RNA target sites: n/a
Primers:
Forward primer: CTGCTGTTCCTCCTCTGATCTGC
Reverse primer: CCGGGAGCTTTACTAAAGCCATACC
WT Protein sequence